study of pod length trait in doubled haploid brassica napus population by molecular markers

نویسندگان

h. samizadeh

b. yazdi-samadi

m. r. bihamta

a. taleii

g. r. stringam

چکیده

pod length is one of the effective components on canola yield selection on the basis of which can increase not only the seed yield but also the oil yield. molecular markers asso-ciated with long pod loci were identified in a doubled haploid population derived from a cross between the canola lines ‘quantum’ (long pod) ‘china a’ (short pod) using rapd and bulked segregant analysis. a molecular marker linkage map of 37 loci for this popu-lation was used to identify quantitative trait loci (qtl) controlling pod length of which two markers in two unlinked loci were selected by using an interval mapping model which explained 22% of phenotypic variation for pod length in this population. selection for markers at two loci for increasing pod length resulted in a group of doubled haploid lines with an average 112 mm pod length that increased 15% of whole population mean. this shows that using these markers in the breeding program will enhance the breeding of long pod canola varieties.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A Major Locus for Manganese Tolerance Maps on Chromosome A09 in a Doubled Haploid Population of Brassica napus L.

Soil acidity poses a major threat to productivity of several crops; mainly due to the prevalence of toxic levels of Al3+ and Mn2+. Crop productivity could be harnessed on acid soils via the development of plant varieties tolerant to phytotoxic levels of these cations. In this study, we investigated the extent of natural variation for Mn2+ tolerance among ten parental lines of the Australian and...

متن کامل

Efficient Doubled Haploid Production in Brassica napus via Microspore Colchicine Treatment in vitro and Ploidy Determination by Flow Cytometry

An efficient microspore culture protocol was used to rapidly produce homozygous doubled haploid (DH) progeny of crosses between low erucic canola and high erucic resynthesized rapeseed (Brassica napus L.). Microspores of Canadian cultivars ‘Excel’ and ‘Profit’ as well as three F1 hybrids with the resynthetic line RS239 were treated with colchicine immediately after isolation. Flow cytometry was...

متن کامل

Hormones and Pod Development in Oilseed Rape (Brassica napus).

The endogenous levels of several plant growth substances (indole acetic acid, IAA; abscisic acid, ABA; zeatin, Z; zeatin riboside, [9R]Z; isopentenyladenine, iP; and isopentenyladenosine, [9R]iP were measured during pod development of field grown oilseed Rape (Brassica napus L. var oleifera cv Bienvenu) with high performance liquid chromatography and immunoenzymic (enzyme-linked immunosorbent a...

متن کامل

Linkage analysis of molecular markers and quantitative trait loci in populations of inbred backcross lines of Brassica napus L.

Backcross populations are often used to study quantitative trait loci (QTL) after they are initially discovered in balanced populations, such as F(2), BC(1), or recombinant inbreds. While the latter are more powerful for mapping marker loci, the former have the reduced background genetic variation necessary for more precise estimation of QTL effects. Many populations of inbred backcross lines (...

متن کامل

Comparative quantitative trait loci for silique length and seed weight in Brassica napus

Silique length (SL) and seed weight (SW) are important yield-associated traits in rapeseed (Brassica napus). Although many quantitative trait loci (QTL) for SL and SW have been identified in B. napus, comparative analysis for those QTL is seldom performed. In the present study, 20 and 21 QTL for SL and SW were identified in doubled haploid (DH) and DH-derived reconstructed F2 populations in rap...

متن کامل

A study of genetic diversity among Brassica napus and Brassica juncea germplasm collections using Simple Sequence Repeat (SSR) molecular markers

Brassica species represent a broad range of crops. This reflects the high degree of genetic diversity and related phenotypic plasticity. An understanding of the genetic basis of Brassica diversity aids both breeding and the discovery of rare alleles and traits. Single Sequence Repeat (SSR) molecular markers are popular tools for assessing genetic diversity due to their high degree of polymorphi...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
journal of agricultural science and technology

ناشر: tarbiat modares university

ISSN 1680-7073

دوره 9

شماره Number 2 2010

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023